In brief, one microliter of the 50 µL isolated RNA was then subjected to a 20 µL reverse transcription using miR-21 stem-loop reverse primer or oligo dT primer. Relative expression of miR-21 (Panel A), and S15 (Panel B) was determined by RT-qPCR of total RNA samples. Total RNA was isolated from 10 to 100,000 HeLa cells using Norgen's Total RNA Purification Kit ( blue), a competitors silica-based kit ( green) and a phenol-based RNA extraction method ( red). Norgens Total RNA Purification Kit allows consistent isolation of both large and small RNA from different input amounts. Linear and Sensitive Isolation of Both Large and Small RNA. Top 5 Reasons to Choose Norgen's RNA Extraction Technologyįigure 5. Ultracentrifugation, Exoquick, Filtration Cat. Isolate RNA after Purifying EVs and Exosomes Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses. This 96-well kit provides a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. miRNA), the column binds small RNAs regardless of their GC content. Moreover, when the RNA sequences are small (e.g. Isolate all RNA sequences at an equal rate irrespective of size. These kits purify all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol.

Extract high quality and purity RNA with excellent RIN values and A 260/A 280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. These kits are suitable for the isolation of total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more.

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